Abstract: When carrying out hydrogeological investigation and evaluation, it is of great significance to conduct stratified pumping test for each water-filled aquifer and accurately obtain hydrogeological parameters of aquifer.Taking H40310 borehole of Hujiahe Coal Mine in Binchang Mining Area as the research object, combined with the advantages of Packer sealing layered water stopping technology, on the basis of summarizing and analyzing the principle of multi-aquifer same diameter water stopping technology, through the design of pumping test scheme, the research on the use method and applicable conditions of the same diameter water stopping is carried out.The results show that the double embolization system can achieve the purpose of stratified pumping and sampling at any depth in the same diameter borehole, and the test effect is good.The permeability coefficient of the whole section of the Luohe Formation is 0.276 4 m/d.The permeability coefficient obtained by the same diameter water stopping pumping test is consistent with the permeability coefficient in the region, indicating that the results of the same diameter water stopping pumping test are credible.The use of expansion hose(airbag)water stopper with the same diameter can greatly reduce the pore size, avoid multiple reaming in the construction of hydrological hole, and the water stopping quality is reliable.